RNA
GreenFET1T

Part:BBa_K1586001:Design

Designed by: Exeter iGEM 2015   Group: iGEM15_Exeter   (2015-09-10)


Synthetic toehold riboswitch - T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751


Design Notes

The first 12 nucleotides of the toehold (not including the GGG leader sequence) should not form a part of the toehold stem. The majority of the RBS should be within the loop region of the toehold. There must not be an inframe stop codon within the main linker sequence. The linker sequence length must be a multiple of three.


Source

The sequence of this part was designed by and characterised in cells by Green et al. 2014 (http://www.sciencedirect.com/science/article/pii/S0092867414012896; supplementary information, table 3, Forward Engineered Toehold 1).

References